Off-campus WSU users: To download campus access dissertations, please use the following link to log into our proxy server with your WSU access ID and password, then click the "Off-campus Download" button below.

Non-WSU users: Please talk to your librarian about requesting this thesis through interlibrary loan.

Access Type

WSU Access

Date of Award

January 2019

Degree Type


Degree Name



Biomedical Engineering

First Advisor

Mai Lam


Chronic hyperglycemia is a known risk factor in type 1 diabetes (T1D) including pancreatic beta, renal and retinal cells. The transcriptional and tumor repressor, Thioredoxin-interacting protein (TXNIP), is upregulated in diabetes and by high glucose in various tissues. TXNIP binds to and inhibits the anti-oxidant capacity of thioredoxin, causing oxidative stress and cell death. This process plays a causative role in diabetic complications. We investigated if the TXNIP-promoter can be linked with a therapeutic gene such as insulin and induce their expression under diabetic conditions. This study characterized the TXNIP-promoter linked genes when co-transfected into adipose derived mesenchymal stem cells (ASCs). The results of the duel transfected ASCs with the TXNIP-promoter, PDX1 and insulin show a decrease in cellular apoptosis in ATP and MTT assays. This characterization study has important implications towards understanding the mechanism of the TXNIP-promoter’s cellular function and a future potential for reducing chronic hyperglycemia in diabetics.

Off-campus Download