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Access Type
WSU Access
Date of Award
January 2019
Degree Type
Thesis
Degree Name
M.S.
Department
Biomedical Engineering
First Advisor
Mai Lam
Abstract
Chronic hyperglycemia is a known risk factor in type 1 diabetes (T1D) including pancreatic beta, renal and retinal cells. The transcriptional and tumor repressor, Thioredoxin-interacting protein (TXNIP), is upregulated in diabetes and by high glucose in various tissues. TXNIP binds to and inhibits the anti-oxidant capacity of thioredoxin, causing oxidative stress and cell death. This process plays a causative role in diabetic complications. We investigated if the TXNIP-promoter can be linked with a therapeutic gene such as insulin and induce their expression under diabetic conditions. This study characterized the TXNIP-promoter linked genes when co-transfected into adipose derived mesenchymal stem cells (ASCs). The results of the duel transfected ASCs with the TXNIP-promoter, PDX1 and insulin show a decrease in cellular apoptosis in ATP and MTT assays. This characterization study has important implications towards understanding the mechanism of the TXNIP-promoter’s cellular function and a future potential for reducing chronic hyperglycemia in diabetics.
Recommended Citation
Serra, Viktoriah, "Characterization Of Novel Thioredoxin Interaction Protein Promoter By Using Adipose Derived Mesenchymal Stem Cells" (2019). Wayne State University Theses. 719.
https://digitalcommons.wayne.edu/oa_theses/719