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Access Type
WSU Access
Date of Award
January 2025
Degree Type
Dissertation
Degree Name
Ph.D.
Department
Cancer Biology
First Advisor
Gerold Bepler
Abstract
We discovered a novel interaction between ribonucleotide reductase subunit 1 (R1) and the catalytic minichromosome maintenance proteins (MCM4/6/7) by in vitro binding and co-immunoprecipiation in various mammalian cell lines. We observed that R1 colocalizes with MCM4/6/7 in mitotic cells, and in non-mitotic cells, R1 only co-localizes with MCM7 in the cytosol. When R1 expression is inducibly knocked down in the presence of dNTPs, we see a decline in MCM7 expression and reduced cell proliferation. We surmise that there are two R1-MCM complexes: a cytoplasmic R1-MCM7 complex and an M-phase associated R1-MCM4/6/7 complex. Our work has demonstrated that R1 stabilizes MCM7 which underscores a secondary R1 function that is independent of its role in ribonucleotide reductase.
Recommended Citation
Malysa, Agnes, "Interaction Of The Regulatory Subunit Of Ribonucleotide Reductase With Minichromosome Maintenance Complexes Regulate Cell Growth" (2025). Wayne State University Dissertations. 4165.
https://digitalcommons.wayne.edu/oa_dissertations/4165