Human Biology Open Access Pre-Prints

Eva D. Juárez Cortés, Laboratorio de Histocompatibilidad, Banco Central de Sangre, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Miguel A. Contreras Sieck, Laboratorio de Genética Molecular, Escuela Nacional de Antropología e Historia, Mexico City, Mexico
Agustín J. Arriaga Perea, Laboratorio de Histocompatibilidad, Banco Central de Sangre, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Rosa M. Macías Medrano, Laboratorio de Histocompatibilidad, Banco Central de Sangre, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Anaí Balbuena Jaime, Departamento de Trasplantes, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Paola Everardo Martínez, Laboratorio de Fisiología, Bioquímica y Genética, Escuela Nacional de Antropología e Historia, Mexico City, Mexico
Joaquín Zúñiga, Departamento de Inmunología, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City, Mexico
Víctor Acuña Alonzo, Laboratorio de Genética Molecular, Escuela Nacional de Antropología e Historia, Mexico City, Mexico
Julio Granados, Departamento de Trasplantes, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Rodrigo Barquera, Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Jena, GermanyFollow

Document Type

Open Access Preprint

Anticipated Volume

89

Anticipated Issue

3

Abstract

The major histocompatibility complex is directly involved in the immune response and thus the genes coding for its proteins are useful markers for the study of genetic diversity, susceptibility to disease (autoimmunity and infections), transplant medicine, and pharmacogenetics, among others. The polymorphism of the system also allows researchers to use it as a proxy for population genetics analysis, such as genetic admixture and genetic structure. In order to determine the immunogenetic characteristics of a sample from the northern part of Mexico City and to use them to analyze the genetic differentiation from other admixed populations, including those from previous studies of Mexico City population, we analyzed molecular typing results of donors and patients from the Histocompatibility Laboratory of the Central Blood Bank of the Centro Médico Nacional La Raza selected according to their geographic origin. HLA-A, -B, -DRB1, and -DQB1 alleles were typed by PCR-SSP procedures. Allelic and haplotypic frequencies, as well as population genetics parameters, were obtained by maximum likelihood methods. The most frequent haplotypes found included HLA-A*02/-B*39/- DRB1*04/-DQB1*03:02P; HLA-A*02/-B*35/-DRB1*04/-DQB1*03:02P; HLA-A*68/-B*39/- DRB1*04/-DQB1*03:02P, and HLA-A*02/-B*35/-DRB1*08/-DQB1*04. Important to observe is that the second most frequent haplotype found in our sample (HLA-A*02/-B*35/-DRB1*04/- DQB1*03:02P) has not been previously reported in any mixed ancestry populations from Mexico but it is commonly encountered in Native American human groups, which can be a reflection on the impact of migration dynamics in the genetic conformation of the northern part of Mexico City, and the limitations of previous studies with regard to the genetic diversity of the analyzed groups. Differences found in haplotypic frequencies demonstrated that large urban conglomerates cannot be analyzed as one homogeneous entity, but rather should be understood as a set of structures in which social, political, and economical factors influence their genesis and dynamics.

Additional Files
HB-89.3_Cortes_Supplemental_Table_1_Updated.doc (181 kB)
Supplementary Table 1. Complete Description and References of the Populations used in the Principal Components Analysis (PCA)
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