Research Mentor Name
Research Mentor Email Address
Institution / Department
Wayne State University School of Medicine Department of Psychiatry & Behavioral Neurosciences
Level of Research
Traumatic brain injury (TBI) is associated with chronic pain and persistent neuroinflammation. Opioids are often prescribed in order to relieve pain symptoms, but recent evidence suggests that their use negatively impacts the neuropathology of TBI leading to behavioral impairments and exacerbated neuroinflammation. The de-ubiquinating enzyme tumor necrosis factor alpha-induced protein (TNFAIP)3 or A20, inhibits the inflammatory signaling transcription factor nuclear factor (NF)-kB leading to attenuation of the inflammatory response. A20-binding inhibitor of nuclear factor kB (ABIN-1), which physically interacts with A20, also plays a role in the inhibition of NF-kB and is poorly researched in the central nervous system (CNS). Additionally, recent evidence suggests that ABIN-1 negatively regulates mu-opioid receptors. Therefore, dysregulation of ABIN-1 may contribute to the neuropathology post-TBI, especially after treatment with opioids. Phase 1 of the project involved determining the influence of neuroinflammation on ABIN-1 expression. Ten-week old male mice were injected intraperitoneally with a single dose (10mg/kg) of lipopolysaccharide (LPS), a potent immunoactivator, or saline (control). Brains were harvested 24 hours after injection for immunoblotting and co-immunoprecipitation assays. Preliminary results suggest that ABIN-1 protein is poorly expressed in the prefrontal cortex, nucleus accumbens, and ventral hippocampus, but that brain levels of ABIN-1 may be modulated by LPS treatment. Future research will investigate ABIN-1 protein expression throughout the mouse brain at 1, 7, and 10 days after a moderate level TBI in the presence or absence of morphine exposure to evaluate the role of ABIN-1 in mediating opioid action in the injured brain.
Medical Cell Biology | Medical Immunology | Medical Neurobiology | Medicine and Health Sciences | Nervous System Diseases | Neurosciences
Orellana, Aaron and Conti, Alana C., "Characterization of ABIN-1 in the Traumatically Injured Brain" (2021). Medical Student Research Symposium. 74.