Research Mentor Name

Dr. Ashok Kumar

Research Mentor Email Address

akuma@med.wayne.edu

Institution / Department

Department of Ophthalmology, Visual and Anatomical Sciences

Document Type

Research Abstract

Research Type

basicbio

Level of Research

no

Abstract

Purpose: To evaluate the effects of viscous lidocaine gel on the antimicrobial efficacy of povidone-iodine (PI) and their order of application in both in vitro and in vivo models.

Methods: In vitro antibacterial effects were tested against Staphylococcus aureus (S. aureus) with disc diffusion methods for application of lidocaine alone, PI alone, PI before lidocaine, and lidocaine before PI. Zones of inhibition were measured after incubation at 37°C overnight. Mouse eyes were colonized with S. aureus for in vivo study to which PI and/or lidocaine were applied in various combinations. Eyes were then rinsed with saline, and the runoff fluid was collected, diluted, and plated on agar. Viable bacterial estimation was performed after incubation overnight at 37°C.

Results: In vitro studies demonstrated a significantly smaller (PP>0.05). In vivo studies showed that mouse eyes treated with lidocaine prior to PI had significantly more (PS. aureus growth compared to eyes that had PI applied prior to lidocaine.

Conclusions: Both in vitro and in vivo studies demonstrated that lidocaine gel interfered with PI’s antiseptic properties when placed between the surface and PI. Our results are consistent with previous in vitro studies and provide greater evidence for applying PI before lidocaine gel if a viscous anesthetic agent is used.

Disciplines

Bacteria | Bacterial Infections and Mycoses | Eye Diseases | Medical Microbiology | Medicine and Health Sciences | Ophthalmology

Comments

This work is supported by KEI-TRIG award to Dr. Aleksey Mishulin and the Wayne State University School of Medicine Medical Student Research Fellowship (MSRF) to Cecilly Kelleher and Dr. Ashok Kumar.

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