Research Mentor Name

Dr. Qing-Sheng Mi

Research Mentor Email Address

qmi1@hfhs.org

Institution / Department

Henry Ford Health System

Document Type

Research Abstract

Research Type

clinicalresearch

Level of Research

no

Abstract

Cutaneous psoriasis (PsC) is an auto-immune disorder affecting 60 million people globally, among 30% of whom progress to psoriatic arthritis (PsA), a disease with poorly understood etiology, making diagnosis and treatment difficult. Indeed, a complete systemic immune profile of PsA has yet to be performed. In the study herein, we collected peripheral blood samples from patients with PsC, PsA with or without systemic therapy, and healthy controls (HC), and utilized mass cytometry by time of flight (CyTOF) to acquire immune cell profiles of major leukocyte subsets. We found that patients with PsC and/or PsA exhibited increased frequencies of intermediate (CD14+CD16+) and nonclassical (CD14-CD16+) monocytes as well as regulatory T cells. Separation of our heterogenous patient population revealed distinct immune profiles according to ethnicity and sex in patient groups. Analysis of homing markers revealed upregulation of CCR4, CCR7, and CXCR3 on Classical Monocytes and/or Naïve CD8+ T cells in PsC and/or PsA patients. Moreover, analysis of functional markers revealed upregulation of CD38, CD28, and CD25 on Tregs and EM CD4+ T cells in PsC and/or PsA patients. Unbiased machine learning algorithms (CITRUS) revealed upregulation of Classical Monocytes in PsC and PsA compared to HC patients. Lastly, CITRUS revealed upregulated Intermediate Monocytes in PsA compared to PsC patients, and upregulated Classical Monocytes in treated PsA compared to untreated PsA patients. Therefore, we provide a comprehensive profile of immune cell population frequencies and phenotypes in patients with PsC and PsA, highlighting Monocytes and Tregs as potential biomarkers for early diagnosis of PsA.

Disciplines

Medicine and Health Sciences

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