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Access Type
WSU Access
Date of Award
January 2022
Degree Type
Thesis
Degree Name
M.S.
Department
Anatomy and Cell Biology
First Advisor
Bruce Berkowitz
Abstract
Purpose: Pharmaceutically correcting mitochondrial dysfunction may mitigate outer retina degenerative disease but evaluating treatment efficacy in vivo remains challenging. Here, we test the hypothesis that outer retina imaging energy biomarkers are sensitive to mitochondrial-activating drugs.Methods: Studies were performed in wildtype mice. We measured the inner segment ellipsoid zone (ISez) profile shape, external limiting membrane – retinal pigment epithelium (ELM-RPE) thickness and, hyporeflective band (HB) intensity. Results: To check biomarker reproducibility, we first examined a paired test-retest design in the same mouse. Unexpectedly, the rod energy signature increased linked with handling stress. A cross-sectional design allowed reliable detection of light-exposure duration and anesthesia-evoked increases in the rod energy signature. Changes in rod bioenergetics biomarkers in wildtype mice were only detectable at high doses of a phosphodiesterase inhibitor and not with a mitochondrial uncoupler. Conclusion: The results herein set the stage for evaluating drug-based improvements in rod bioenergy biomarkers during rod degeneration.
Recommended Citation
Katz, Ryan, "Testing Drug Induced Improvements In Outer Retina Mitochondria Activity Based On Optical Coherence Tomography Biomarkers" (2022). Wayne State University Theses. 998.
https://digitalcommons.wayne.edu/oa_theses/998