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Access Type

WSU Access

Date of Award

January 2017

Degree Type

Thesis

Degree Name

M.S.

Department

Nutrition and Food Science

First Advisor

Kequan Zhou

Abstract

Obesity is a global epidemic which has received increasingly more attention in recent years. Studies have suggested that the distribution of gut microbiota influences the development of obesity. Probiotics have a physiological function that assists in improving the microbial ecosystem. Eubacterium Siraeum has been found to have a negative correlation with obesity, which indicates that Eubacterium Siraeum may become a potential probiotic. First, it is crucial to batch culture to explore their characteristics and underlying functions for further study. In addition, we need to solve the problem associated with low viability of bacteria via GIT. This study mainly focused on investigating the optimal condition for the growth of Eubacterium Siraeum and testing a common protective method on E. siraeum. Environmental conditions were tested to find the optimal one. Comparing the viability of bacterial freeze-dried powder with those of encapsulated bacteria was to test the protective effectiveness from GI tract. In this study, we found that the optimal temperature was 35℃, E. siraeum also can grow equally well at 37℃ and 40°C, optimal pH is 6, E. siraeum can grow at pH ranging from 5 to 7. IMO and GOS are best prebiotic for the growth E. siraeum. Alginate-based encapsulation technique has an encouraging protective effect on E. siraeum.

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