Access Type
Open Access Thesis
Date of Award
January 2017
Degree Type
Thesis
Degree Name
M.S.
Department
Biochemistry and Molecular Biology
First Advisor
Zhe Yang
Abstract
Methylation of histones and non-histone proteins play vital roles in numerous cellular processes including gene expression regulation and DNA damage response. The identifications of methyltransferase SMYD protein family are not well characterized. SMYD5 is a unique and critical member of SMYD family involved in immune response, stem cell renew, hematopoiesis regulation and cancer metastasis. Understanding its function and structure is monumental to human disease. With the achievement of SMYD5 expression and purification, the association between SMYD5 and its substrate histone H4 has been investigated. While possessing a poly-E tail instead of the TPR domain included in other members, SMYD5 is assumed that associated with Calcium and could potentially be a mitochondrial protein.
Recommended Citation
Xue, Wen, "Expression, Purification And Characterization Of Lysine Methyltransferase Smyd5" (2017). Wayne State University Theses. 596.
https://digitalcommons.wayne.edu/oa_theses/596