Access Type

Open Access Thesis

Date of Award

January 2017

Degree Type

Thesis

Degree Name

M.S.

Department

Nutrition and Food Science

First Advisor

Smiti Gupta

Abstract

Alzheimer's disease (AD) is the most widely recognized reason for dementia in the aging population. It is described by intellectual decay and deposition of ß-amyloid plaques in the hippocampus. It has been demonstrated that hypercholesterolemia incited by elevated cholesterol abstain from food is related with AD advancement. Expanded level of oxidative stress has additionally been seen in AD patients. An essential methodology to treat or postpone the disability depends on dietary change, utilizing nourishment supplements. OPP, a water-soluble fraction from oil palm fruit, rich in phenolics has been found to possess significant antioxidant activities. Curcumin, a polyphenol extricated from the plant Curcuma longa, has demonstrated its remedial advantages in Alzheimer's ailment and was utilized as a positive control. Our results demonstrated the dietary cholesterol actuated hypercholesterolemia which expanded AD-like pathological changes in matured rats including β-amyloid amassing and psychological decrease. OPP & curcumin attenuate the process of AD for their antioxidant and anti-inflammatory effects by improving these pathological changes. acquire the spectrum of samples. Multivariate analysis software, SIMCA-P+, was applied to demonstrate the differences in plasma 1H NMR profiles among the groups. Partial least Squares (PLS-DA) score plots showed clear separation among all four groups indicating differences in their metabolomics profiles at the end point. OPLS regression analysis gave significant correlations between the urinary metabolomic profiles and the β amyloid burden. The metabolites responsible for the differences in the metabolomic profile among groups were then quantified using CHENOMX NMR metabolite database. Some metabolites from the homocysteine metabolism pathway were significantly altered in the cholesterol fed group (H) as compared to the treatment groups (HP, HC). Treatment with curcumin (HC) or OPP (HP) modulated the concentration of these metabolites closer to the control levels. This pathway has been shown to be perturbed in neurodegenerative diseases. Taken together, curcumin exhibited a potential therapeutic effect in high cholesterol diet induced AD. Moreover, specific plasma metabolites may serve as non-invasive biomarkers for progression of neurodegenerative diseases including AD.

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