Access Type
Open Access Dissertation
Date of Award
January 2024
Degree Type
Dissertation
Degree Name
Ph.D.
Department
Chemistry
First Advisor
Jeremy J. Kodanko
Abstract
Metal complexes have many advantages as biochemical tools due to their rich photophysical properties and unique geometry. Metal-based complexes are commonly reported as emissive probes or photoactivatable biological agents. Long emission lifetimes of metal complexes allow for the use of lifetime gating which eliminates autofluorescence found in assays, a distinct advantage over short-lived organic fluorophores. This work utilizes this powerful technique to generate reversible, emissive probes for monitoring CYP3A4 active site occupancy. In addition to the emissive properties of metals, metals can also be used as photodynamic therapy (PDT) and photochemotherapy (PCT) agents. These light-activated strategies provide important spatiotemporal control which grants localized treatment and elimination of off-target side effects found in conventional therapeutics. This work represents advances in the PCT/PDT field to shift to use of green-light activated agents, opposed to commonly found blue-light agents associated with poor tissue penetration and light toxicity. Together, this collection of work demonstrates the superiority of the photophysical properties of metal complexes and its promising biological applications
Recommended Citation
Denison, Madeline, "Exploiting Light To Probe Biological Processes" (2024). Wayne State University Dissertations. 4092.
https://digitalcommons.wayne.edu/oa_dissertations/4092
