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Access Type
WSU Access
Date of Award
January 2024
Degree Type
Dissertation
Degree Name
Ph.D.
Department
Biological Sciences
First Advisor
Victoria H. Meller
Abstract
Drosophila melanogaster males increase expression from their single X chromosome to match that of the two female X’s. This requires localization of the Male Specific Lethal (MSL) complex to X linked genes and modification of chromatin by this complex. How X chromatin is selectively identified remains unclear, but cis-acting recruiting elements are involved. Chromatin Entry Sites (CES) bind an adapter protein to recruit the MSL complex directly. A class of AT-rich satellite repeats, the 1.688X repeats, are enriched on the X and facilitate compensation of nearby genes. How these elements cooperate to identify the X is unknown Chromatin state and nuclear architecture influence gene expression and dosage compensation in many organisms. In this study we explore the role of nuclear architecture factors in recognition of the male X chromosome. Genetic interactions with mutations reducing MSL localization indicated that HP2, SAF-A, ISWI, D1, Nup153 and Cp190 participate in dosage compensation. We used a reporter for recruitment to determine that HP2 and SAF-A exert non-specific effects on reporter expression. In contrast, ISWI and D1 contribute to recruitment by the CES and Cp190 influences recruitment only at 1.688X repeats. Our findings reveal that distinct aspects of chromatin organization and nuclear architecture contribute to recruitment of dosage compensation by the CES and 1.688X repeats. We postulate that recruiting elements cooperatively mark the X by engaging multiple aspects of nuclear organization.
Recommended Citation
Lauria Sneideman, Maggie, "Drosophila Melanogaster X Chromosome Recruiting Elements Employ Nuclear Architecture For Msl Localization" (2024). Wayne State University Dissertations. 4044.
https://digitalcommons.wayne.edu/oa_dissertations/4044
