Access Type
Open Access Dissertation
Date of Award
January 2024
Degree Type
Dissertation
Degree Name
Ph.D.
Department
Chemistry
First Advisor
Thomas H. Linz
Abstract
ABSTRACTDEVELOPMENT OF MICROFLUIDIC THERMAL GEL ELECTROPHORESIS FOR BIOLOGICAL ANALYSIS by MARIO AMBROCIO CORNEJO May 2024 Advisor: Dr. Thomas H. Linz Major: Chemistry (Analytical Chemistry) Degree: Doctor of Philosophy The molecular biology that takes place within cells is vital to the over function of an organism. Healthy cellular function is crucial to a wide web of interconnected processes that need to be balanced and regulated. When these processes become abnormal, disease states are developed. The mapping of biological networks has led to the development of gene therapy and more accurate diagnostic tests. However, there are still vital biomolecules that due to their physical properties are difficult to analyze. In this dissertation, microfluidic thermal gel electrophoresis (TGE) has been developed for cell enrichment, analysis of cellular content (specifically for microRNAs), and improved selectivity by incorporating high-temperature analysis. These methods were all developed with the goal of improving upon cellular analysis by reducing sample dilution, improving upon limit of detection, and preventing probe promiscuity during multiplex analysis. Microfluidic TGE is also developed to perform label-free detection to reduce sample preparation times and cost. We have demonstrated a wide range of compatibility from TGE analysis and made fundamental characterizations needed for future method developments
Recommended Citation
Cornejo, Mario Ambrocio, "Development Of Microfluidic Thermal Gel Electrophoresis For Biological Analysis" (2024). Wayne State University Dissertations. 3968.
https://digitalcommons.wayne.edu/oa_dissertations/3968
