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Access Type

WSU Access

Date of Award

January 2018

Degree Type

Dissertation

Degree Name

Pharm.D./Ph.D.

Department

Chemistry

First Advisor

Matthew J. Allen

Abstract

The research described in this thesis was focused on detection of EuII and EuIII using different

modes of imaging, such as 19F-magnetic resonance imaging and photoacoustic imaging within the

context of oxygen-sensitive imaging agents. Prior to this research, EuII had been explored as a T1

shortening contrast agent for magnetic resonance imaging, but the optical properties of EuII had

never been explored within the context of an oxidation-responsive imaging agent, and a redox

responsive 19F- magnetic resonance imaging signal had not been detected in vivo.

A fluorinated EuII-containing complex was used to evaluate positive contrast intraperitoneal

until it was oxidized to the corresponding EuIII-containing complex in vivo and detected with 19F

magnetic resonance imaging. Additionally, oxidized EuIII-containing complex was detected intact

in mouse urine, which is promising for future toxicity studies and use in vivo. Two EuII-containing

complexes that absorb visible light, were detected using photoacoustic imaging until oxidized to

the EuIII-containing analogs which turns the photoacoustic signal off.

The ability to form unique images EuII/III-containing complexes as a function of oxidation state

is a powerful tool to distinguish regions of hypoxia in vivo.

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