Access Type
Open Access Dissertation
Date of Award
January 2018
Degree Type
Dissertation
Degree Name
Ph.D.
Department
Chemistry
First Advisor
YOUNG-HOON AHN
Second Advisor
JOHN SANTALUCIA
Abstract
ABSTRACT
CARBOHYDRATE-BASED INDUCERS OF CELLULAR STRESS FOR TARGETING CANCER CELL METABOLISM
by
FIDELIS TOLOYI NDOMBERA
May 2018
Advisor: Dr. Young-Hoon Ahn
Major: Chemistry (Biochemistry)
Degree: Doctor of Philosophy
Metabolic reprogramming and redox control of cancer cells is vital for their proliferation, but also provides selective strategies for treating cancer. Increased generation of reactive oxygen species (ROS) and an intricate control of redox status in cancer cells relative to normal cells provide a basis for designing ROS-inducing anticancer agents. In my work, I designed, synthesized and evaluated carbohydrate-based small molecules for ROS-generation, cytotoxicity and redox signaling and stress response. Our data from assays, including cell viability assays, enzyme inhibition studies, Western blot studies, click chemistry, and metabolomics, reveal that our compound (K8A) is more potent than 2-DG, a well-known carbohydrate-derived inhibitor of glycolysis. We reported discovery of carbohydrate-based small molecules with the property of blocking altered metabolic activity and enhancing ROS with potential therapeutic benefits for targeting cancer cells. Importantly, we investigated the mechanism of action of our potent compound that involve disruption of protein glycosylation in cancer with downstream effects on cancer metabolism.
Recommended Citation
Ndombera, Fidelis, "Carbohydrate-Based Inducers Of Cellular Stress For Targeting Cancer Cell Metabolism" (2018). Wayne State University Dissertations. 1951.
https://digitalcommons.wayne.edu/oa_dissertations/1951
Included in
Biochemistry Commons, Cell Biology Commons, Chemistry Commons