Document Type
Article
Abstract
The effects of a single bout of resistance exercise (RE) in conjunction with peri-exercise branched chain amino acid (BCAA) and carbohydrate (CHO) ingestion on skeletal muscle signaling markers indicative of muscle protein synthesis (MPS) were determined. It was hypothesized that CHO + BCAA would elicit a more profound effect on these signaling markers compared to CHO. Twenty-seven males were randomly assigned to CHO, CHO + BCAA, or placebo (PLC) groups. Four sets of leg presses and leg extensions were performed at 80% 1RM. Supplements were ingested 30 min and immediately prior to and after RE. Venous blood and muscle biopsy samples were obtained immediately prior to supplement ingestion and 0.5 hr, 2 hr, and 6 hr after RE. Serum insulin and glucose and phosphorylated levels of muscle insulin receptor substrate 1 (IRS1), protein kinase B (Akt), mammalian target of rapamycin (mTOR), p70S6 kinase (p70S6K), and 4E binding protein 1 (4E-BP1) were assessed. Data were analyzed by two-way repeated measures ANOVA. Significant group x time interactions were observed for glucose and insulin (p < 0.05) showing that CHO and CHO + BCAA were significantly greater than PLC. Significant time main effects were observed for IRS1 (p = 0.001), Akt (p = 0.031), mTOR (p = 0.003), and p70S6K (p = 0.001). CHO and CHO + BCAA supplementation significantly increased IRS-1 compared to PLC (p = 0.002). However, peri-exercise co-ingestion of CHO and BCAA did not augment RE-induced increases in skeletal muscle signaling markers indicative of MPS when compared to CHO.
Disciplines
Dietetics and Clinical Nutrition | Exercise Physiology | Human and Clinical Nutrition
Recommended Citation
Ferreira, M. P., Li, R., Cooke, M., Kreider, R. B., and Willoughby, D. S. Peri-exercise co-ingestion of branched-chain amino acids and carbohydrate in men does not preferentially augment resistance exercise-induced increases in PI3K/Akt-mTOR pathway markers indicative of muscle protein synthesis. Nutrition Research (2014). doi: 10.1016/j.nutres.2013.12.007
Author's unformatted final accepted manuscript
nr.2014.tables.unformatted.pdf (85 kB)
Author's unformatted tables
Included in
Dietetics and Clinical Nutrition Commons, Exercise Physiology Commons, Human and Clinical Nutrition Commons
Comments
NOTICE IN COMPLIANCE WITH PUBLISHER POLICY: This is the author’s final manuscript version, post-peer-review, of a work accepted for publication in Nutrition Research. Changes resulting from the publishing process may not be reflected in this document; changes may have been made to this work since it was submitted for publication. This version has been formatted for archiving (including pagination); a definitive version is scheduled for publication in Nutrition Research (2014) http://dx.doi.org/10.1016/j.nutres.2013.12.007