Document Type



Control of growth and differentiation during mammalian embryogenesis may be regulated by growth factors from embryonic or maternal sources. With the use of single-cell messenger RNA phenotyping, the simultaneous expression of growth factor transcripts in single or small numbers of preimplantation mouse embryos was examined. Transcripts for platelet-derived growth factor A chain (PDGF-A), trans- forming growth factor (TGF)-a, and TGF- 1, but not for four other growth factors, were found in whole blastocysts. TGF-cx, TGF-(31, and PDGF antigens were detected in blastocysts by immunocytochemistry. Both PDGF-A and TGF-a were detected as maternal transcripts in the unferilized ovulated oocyte, and again in blastocysts. TGF- P1 transcripts appeared only after fertilization. The expression of a subset of growth factors in mouse blastocysts suggests a role for these factors in the growth and differentiation of early mammalian embryos.


Obstetrics and Gynecology


Copyright (1988) American Association for the Advancement of Science. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Association for the Advancement of Science. This article is the publisher’s version (American Association for the Advancement of Science), previously appearing in SCIENCE, (1988, 241). Also available online at