In this study, we report for the first time that both t-SNAREs and v-SNARE and their complexes in buffered suspension, exhibit defined peaks at CD signals of 208 and 222 nm wavelengths, consistent with a higher degree of helical secondary structure. Surprisingly, when incorporated in lipid membrane, both SNAREs and their complexes exhibit reduced folding. In presence of NSF-ATP, the SNARE complex disassembles, as reflected from the CD signals demonstrating elimination of α-helices within the structure.
Biochemistry | Molecular Biology
Cho WJ, Lee JS, Zhang L, et al. Membrane-directed molecular assembly of the neuronal SNARE complex. Journal of Cellular and Molecular Medicine. 2011; 15: 31-7. doi:10.1111/j.1582-4934.2010.01152.x