N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging

Authors

Shanta R. Bhattarai, Wayne State UniversityFollow
Remant B. Kc, Chonbuk National UniviversityFollow
Sun Y. Kim, School of Medicine, Chonbuk National UniviversityFollow
Manju Sharma, School of Medicine, Chonbuk National UniviversityFollow
Myung S. Khil, Chonbuk National UniviversityFollow
Pyoung H. HwangFollow
Gyung H. Chung, School of Medicine, Chonbuk National UniviversityFollow
Hak Y. Kim, Chonbuk National UniviversityFollow

Document Type

Article

Abstract

Abstract

This project involved the synthesis of N-hexanoyl chitosan or simply modified chitosan (MC) stabilized iron oxide nanoparticles (MC-IOPs) and the biological evaluation of MC-IOPs. IOPs containing MC were prepared using conventional methods, and the extent of cell uptake was evaluated using mouse macrophages cell line (RAW cells). MC-IOPs were found to rapidly associate with the RAW cells, and saturation was typically reached within the 24 h of incubation at 37°C. Nearly 8.53 ± 0.31 pg iron/cell were bound or internalized at saturation. From these results, we conclude that MC-IOPs effectively deliver into RAW cells in vitro and we also hope MC-IOPs can be used for MRI enhancing agents in biomedical fields.

Disciplines

Pharmacy and Pharmaceutical Sciences

Recommended Citation

Bhattarai et al. Journal of Nanobiotechnology 2008, 6:1
doi:10.1186/1477-3155-6-1