Research Mentor Name
Nardhy Gomez-Lopez
Research Mentor Email Address
ngomezlo@med.wayne.edu
Institution / Department
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS)
Document Type
Research Abstract
Research Type
basicbio
Level of Research
no
Abstract
Objective: To determine if bacteria (Ureaplasma parvum and Sneathia spp.) associated with intra-amniotic infection can trigger the induction of cytokine Thymic stromal lymphopoietin (TSLP) in human amnion epithelial cells (hAECs) in vitro.
Material or subjects: Amniotic fluid and chorioamniotic membrane (CAM) were collected from women with sPTL who delivered at term (n=30) or preterm without intra-amniotic inflammation (n=34), with sterile intra-amniotic inflammation (SIAI, n=27), or with intra-amniotic infection (IAI, n=17). Amnion epithelial cells (AECs), Ureaplasma parvum, and Sneathia spp. were also utilized.
Methods: The expression of TSLP, TSLPR, and IL-7Rα was evaluated in amniotic fluid or CAM by RT-qPCR and/or immunoassays. AECs co-cultured with Ureaplasma parvum or Sneathia spp. were evaluated for TSLP expression by immunofluorescence and/or RT-qPCR.
Results: TSLP was elevated in amniotic fluid of women with SIAI or IAI and expressed by the CAM. TSLPR and IL-7Rα had detectable gene and protein expression in the CAM; yet, CRLF2 was specifically elevated with IAI. While TSLP localized to all layers of the CAM and increased with SIAI or IAI, TSLPR and IL-7Rα were minimal and became most apparent with IAI. Co-culture experiments indicated that Ureaplasma parvum and Sneathia amnii upregulated TSLP expression in AECs.
Conclusions: Ureaplasma parvum and Sneathia spp. triggers induction of TSLP, a central component of the intra-amniotic host response during sPTL.
Disciplines
Biology | Cell and Developmental Biology | Cellular and Molecular Physiology | Laboratory and Basic Science Research | Medicine and Health Sciences | Microbiology | Physiology | Translational Medical Research
Recommended Citation
Kanninen, Tomi; Tao, Li; Romero, Roberto; Xu, Yi; Arenas-Hernandez, Marcia; Galaz, Jose; Liu, Zhenjie; Miller, Derek; Levenson, Dustyn; Greenberg, Jonathan M.; Panzer, Jonathan; Padron, Justin; Theis, Kevin; and Gomez-Lopez, Nardhy PhD, "Thymic Stromal Lymphopoietin Participates in the Host Response to Intra-Amniotic Inflammation Leading to Preterm Labor and Birth" (2023). Medical Student Research Symposium. 256.
https://digitalcommons.wayne.edu/som_srs/256
Included in
Biology Commons, Cell and Developmental Biology Commons, Cellular and Molecular Physiology Commons, Laboratory and Basic Science Research Commons, Microbiology Commons, Translational Medical Research Commons