DigitalCommons@WayneState

Title

Co-ingestion of carbohydrate with branched-chain amino acids or L-leucine does not preferentially increase serum IGF-1 and expression of myogenic-related genes in response to a single bout of resistance exercise

Authors

Rui Li, Department of Health Sciences, Northeastern University, Boston MA
Maria Pontes Ferreira, Department of Nutrition & Food Science, Wayne State University, Detroit MIFollow
Matthew B. Cooke, College of Health and Biomedicine, Victoria University, Melbourne, VIC, Australia
Paul La Bounty, Department of Health, Human Performance and Recreation, Baylor University, Waco TX
Bill Campbell, School of Physical Education & Exercise Science, University of South Florida, Tampa FL
Mike Greenwood, Exercise & Sport Nutrition Lab, Department of Health & Kinesiology, Texas A&M University, College Station TX
Darren S. Willoughby, Department of Health, Human Performance and Recreation, Baylor University, Waco TXFollow
Richard B. Kreider, Exercise & Sport Nutrition Lab, Department of Health & Kinesiology, Texas A&M University, College Station TX

Document Type

Article

Abstract

This study determined if the co-ingestion of carbohydrate (CHO) with branched-chain amino acids (BCAA) or L-leucine (LEU) preferentially affected serum IGF-1 and the expression of myogenic-related genes in response to resistance exercise (RE). Forty one, college-age males were randomly assigned to 1 of 4 groups: CHO, CHO-BCAA, CHO-LEU, or placebo (PLC). Resistance exercise consisted of 4 sets of leg press and leg extension at 80% 1RM. Supplements were ingested peri-exercise, and venous blood and muscle biopsies were obtained pre-exercise (PRE), and at 30, 120, and 360 min post-exercise. Serum IGF-1 was determined with ELISA, and skeletal muscle mRNA expression of myostatin, ActRIIB, p21kip, p27kip, CDK2, cyclin B1, cyclin D1, Myo-D, myogenin, MRF-4, and myf5 was determined using real-time PCR. Results were determined with two-way ANOVA for serum IGF-1 and two-way MANOVA for mRNA expression. Serum IGF-1 in CHO and CHO+BCAA was greater than PLC (p < 0.05) but was not affected by RE (p > 0.05). Significant differences were detected between groups for myostatin, ActIIB, MyoD, and myf5 mRNA expression showing CHO to be significantly different than CHO+BCAA, CHO+LEU, and PLC (p < 0.05). At 30, 120 and 360 min post-exercise, p21cip was significantly less than PRE, whereas cyclin D1 was greater than PRE at 120 and 360 min post-exercise (p < 0.05). The co-ingestion of CHO with either BCAA or L-leucine in conjunction with RE had no preferential effect on serum IGF-1 or pre-translational markers indicative of myogenesis.

Disciplines

Dietetics and Clinical Nutrition | Exercise Physiology | Human and Clinical Nutrition

Recommended Citation

Li, R., Ferreira, M. P., Cooke, M. B., La Bounty, P., Campbell, B., Greenwood, M., Willoughby, D. S., & Kreider, R. B. (2015). Co-ingestion of carbohydrate with branched-chain amino acids or L-leucine does not preferentially increase serum IGF-1 and expression of myogenic-related genes in response to a single bout of resistance exercise. Amino Acids 47(6), pp. 1203-1213. http://dx.doi.org/10.1007/s00726-015-1947-8.