Document Type

Article

Abstract

In this study, we report for the first time that both t-SNAREs and v-SNARE and their complexes in buffered suspension, exhibit defined peaks at CD signals of 208 and 222 nm wavelengths, consistent with a higher degree of helical secondary structure. Surprisingly, when incorporated in lipid membrane, both SNAREs and their complexes exhibit reduced folding. In presence of NSF-ATP, the SNARE complex disassembles, as reflected from the CD signals demonstrating elimination of α-helices within the structure.

Disciplines

Biochemistry | Molecular Biology

Comments

This is the author's post-print version, previously appearing in Chemical Physics Letters., 2008 v. 462, p. 6-9. http://www.elsevier.com/