In this study, we report for the first time that both t-SNAREs and v-SNARE and their complexes in buffered suspension, exhibit defined peaks at CD signals of 208 and 222 nm wavelengths, consistent with a higher degree of helical secondary structure. Surprisingly, when incorporated in lipid membrane, both SNAREs and their complexes exhibit reduced folding. In presence of NSF-ATP, the SNARE complex disassembles, as reflected from the CD signals demonstrating elimination of α-helices within the structure.
Biochemistry | Molecular Biology
Cook, Jeremy D.; Cho, Won Jin; Stemmler, Timothy L.; and Jena, Bhanu P., "Self-Assembly and Disassembly of the SNARE Complex: Examined Using Circular Dichroism and Atomic Force Microscopy" (2009). Biochemistry and Molecular Biology Faculty Publications. Paper 6.