The Effect of ACP1-ADA1 Genetic Interaction on Human Life Span

Authors

Nazzareno Lucarini, Laboratory of Human Genetics, School of Biosciences and Biotechnology, Via Gentile III da Varano, University of Camerino, 62032 Camerino, Italy
Valerio Napolioni, Laboratory of Human Genetics, School of Biosciences and Biotechnology, Via Gentile III da Varano, University of Camerino, 62032 Camerino, Italy
Andrea Magrini, Department of Biomedicine and Prevention, Via Montpellier, University of Rome Tor Vergata, 00133 Rome, Italy
Fulvia Gloria, Department of Biomedicine and Prevention, Via Montpellier, University of Rome Tor Vergata, 00133 Rome, Italy

Document Type

Open Access Preprint

Anticipated Volume

84

Anticipated Issue

6

Final Published Version

http://digitalcommons.wayne.edu/humbiol/vol84/iss6/4/

Abstract

Acid phosphatase (ACP1) is a polymorphic enzyme which catalyzes the conversion of flavinmononucleotide (FMN) to riboflavin and regulates the cellular concentration of flavin-adeninedinucleotide (FAD) and, consequently, energy metabolism. Its activity is modulated by adenosine deaminase (ADA1) genotype. Aim of our work is to verify whether individuals with a high proportion of ACP1 f isozyme and carrying ADA*2 allele, displaying the highest phosphatase activity, may have a higher life expectancy. Genomic DNA was extracted from peripheral blood of 569 females and 509 males (18-106 years) randomly recruited from Central Italy. These samples were subdivided into three sexspecific age groups (the ages of women are in square bracket): Class 1:age <66 [<73]; Class 2: age 66-88 [73-91]; Class 3: age >88 [>91]. ACP1 and ADA1 SNPs were genotyped by RFLP-PCR methods and statistical analyses were performed using SPSS 14.0. The results showed a larger proportion of Class 3 individuals displaying high ACP1 f isozyme concen ration and carrying ADA1*2 allele than those of Class 2 and Class 2+1. Thus, we postulate that in Class 3 individuals the high phosphatase activity, resulting from the combined presence of high ACP1 f isozyme concentration and the ADA1*2 allele, lowers the rate of glycolysis which may reduce the amount of metabolic calories and, in turn, activate Sirtuin genes that protect cells against age-related diseases.

Recommended Citation

Open access pre-print, subsequently published as Lucarini, N, Napolioni, V, Magrini, A, and Gloria, F. (2012). "The Effect of ACP₁-ADA₁ Genetic Interaction on Human Life Span," Human Biology 84(6). http://digitalcommons.wayne.edu/humbiol/vol84/iss6/4