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Access Type

WSU Access

Date of Award

January 2022

Degree Type


Degree Name



Biochemistry and Molecular Biology

First Advisor

Jeffrey H. Withey


ABSTRACTGENETIC ANALYSIS OF VIBRIO CHOLERAE DURING ITS HYPERINFECTIOUS STATE IN DANIO RERIO (ZEBRAFISH) by RAVIPAUL SINGH AUGUST 2022 Advisor: Dr. Jeffrey Withey Major: Biochemistry and Molecular Biology Degree: Master of Science Vibrio cholerae is a gram-negative, comma-shaped, monotrichous bacillus and the causative agent of the diarrheal disease cholera. V. cholerae is a natural resident of aquatic environments such as rivers and estuaries and is a highly motile bacterium. Upon entering the human host, V. cholerae travels to its site of colonization in the small intestine and upregulates its virulence factors. Upon escape from the host, V. cholerae can enter a temporal state referred to as hyperinfectivity. The use of the flagellum is critical for each part of V. cholerae’s pathogenesis; the effects of flagellar mutations has not been studied in zebrafish, a natural host for V. cholerae. We hypothesize that whole gene deletions in V. cholerae of key flagellar components such as flhA, flrBC, flgBC, flgOPT, and fliMN would reduce the transmission ability of V. cholerae and prevent it from being hyperinfectious. However, we express that the deletion of flgBC and flgOPT has a minor effect on the colonization ability of V. cholerae, while the flagellar mutants ∆flhA, ∆flgBC, and ∆flgOPT are significantly hindered in their transmission ability compared to wild-type V. cholerae and prevent the bacterium from entering the transient hyperinfectious state.

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