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Access Type

WSU Access

Date of Award

January 2020

Degree Type


Degree Name



Pharmaceutical Sciences

First Advisor

Timothy L. Stemmler


Fe-S clusters are ancient ubiquitous cofactors essential for cell viability. The production and transport of these compounds is not fully understood, but a failure in this pathway can result in the cardio and neuro degenerative disorder known as Friedriech’s ataxia, the most common ataxia with a prevalence of approximately 1:30,000. Recent studies have discovered the presence of the acyl carrier protein within the production complex for Fe-S clusters and a heightened initiative to determine its purpose has arisen since. Using a metal binding competition assay, Differential Scanning Calorimetry, Circular Dichroism, and X-Ray Absorption Spectroscopy we sought to provide a thorough and complete biophysical characterization of both Saccharomyces cerevisiae and Homo sapiens acyl carrier protein orthologs. The resulting data showed the key similarities and differences that are apparent between the two orthologs.

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