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Date of Award
Nutrition and Food Science
EXPLORING THE RELATIONSHIP BETWEEN CELLULAR SENESCENCE AND DNA POLYMERASEβ ΙΝ DOWN SYNDROME
Advisor: Dr. Diane Cress
Major: Nutrition and Food Science
Degree: Master of Science
The goal of this paper is establish and evaluate the relationship between the accelerated aging and premature cellular senescence with the reduced DNA polymeraseβ observed in Down syndrome. Primary fibroblasts from Down syndrome individuals demonstrate greater SA-β-Gal staining (4-fold increase, p<0.001), increased p16 transcript abundance (3-fold increase, p<0.01), and reduced HMGB1 nuclear localization (1.5-fold lower, p<0.01) when compared to primary fibroblast from non-Down syndrome fibroblasts. These biomarkers of senescence establish the premature aging expected in a Down syndrome individual. DNA polymerase β (POLβ) expression is significantly reduced in Down syndrome primary fibroblasts (53% decline, p<0.01). While we are unable to definitely state a direct causal link between DNA polymeraseβ reduction and the precocious aging present, it is demonstrated that DNA polymeraseβ plays a role.
Pace, Brianna Rose, "Investigating The Role Of Dna Polymerase Beta (polb) In The Aging Phenotype Of Down Syndrome" (2017). Wayne State University Theses. 636.