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Date of Award
Sandro da Rocha
Infection of the various forms of Human Papillomavirus can lead to several types of cancer and has been proven to be present in nearly 100% of cervical cancer. Cancer is Cancer caused by HPV is a world-wide health issue. In recent studies, vaccines have shown to be a promising approach in the treatment of cancer. Also previous studies show that the E7-peptide minimum version has antitumor effects when conjugate to nanoparticles and previous studies also show that CpG is a more efficient adjuvant than others commonly used today. The PAMAM dendrimer is commonly used as a vehicle to increase the bioavailability of a drug as well as improve the interaction with APC’s by increasing the lymph node accumulation. We have found that the OH terminated, generation 4 (G4-OH), 8Qmin peptide functionalized PAMAM dendrimer co-delivered with a CpG-B functionalized G4-OH PAMAM dendrimer showed an increase in cellular uptake and increase TLR-9 activation in cell models. In-vivo our drug moiety also showed the ability to enhance the CD8+ t-cell immune response along with an ability to slow tumor growth, decrease overall tumor size and increase life expectancy.
Johns, Joshua, "Cpg-B Odn And E7-Peptide Functionalized Pamam Dendrimers For Co-Delivery Of Hpv-16 Cancers Therapuetic Vaccine" (2017). Wayne State University Theses. 622.