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Access Type

WSU Access

Date of Award

January 2017

Degree Type

Thesis

Degree Name

M.S.

Department

Nutrition and Food Science

First Advisor

Dr. Kequan Zhou

Abstract

ABSTRACT

OPTIMIZATION OF GROWTH CONDITIONS OF “Eubacterium hallii” AS A POTENTIAL PROBIOTICS

by

SAMPURNA GUHATHAKURTA

May 2017

Advisor: Dr. Kequan Zhou

Major: Nutrition and Food Science

Degree: Master of Science

Probiotics are becoming popular to a greater extent over the past two decades for their potential benefits on human health. Therefore, they are widely used in the food industry by incorporation into food products. Nowadays, there are a lot of probiotics which are famous in the market. In this study, the strain of Eubacterium hallii was used because it has a significant importance in the pharmaceutical or food industry especially for treating irritable bowel syndrome and ulcerative colitis. This strain has the property of utilizing lactate and acetate, and produce butyrate. Utilization of lactate helps to keep pH balance of gut epithelium and benefit for other healthy bacteria, while the utilization of acetate reduces metabolic syndrome, obesity, diabetes. The production of butyrate has an anti – inflammatory action. The present objective of the study was to determine the optimum growth conditions. PYG media was used for culturing this strain and it was incubated at 25°C, 37°C, 40°C, 43°C, and 46°C. Optimum growth was obtained at 43°C. Effect of different pH treatment was observed on this strain ranging from pH 7 to pH 2. The growth was significantly different from high pH to low pH. Other growth parameters like bile salt tolerance test was performed to determine the potential of its sustainability in the human gut. The strain was treated with four different concentrations of acetate and it showed to consume a concentration of 66mM of acetate for optimum growth. It was observed to grow at a concentration of 66mM of acetate, therefore for cost effectiveness 66mM can be used. As the concentration was raised to 132 mM, there was a decline in growth because the media was acidic. The least growth was obtained at 33mM concentration of acetate. Ten different prebiotics were used in this study which influenced the growth of the strain, and Gluco-oligosaccharide (GOS) was found to be the best among the prebiotics providing optimum growth. Freeze drying of the strain was performed at 2°C to 8°C and viability of the freeze dried sample was observed. Further investigation should be conducted to determine its potentiality through animal studies.

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