Open Access Thesis
Date of Award
Lori A. Pile
The SIN3A-RPD3 complex components have previously been identified in Drosophila melanogaster. The role of these components in SIN3A function and recruitment was not known. Polytene chromosome analysis following RNAi knockdown was performed to determine if any of the complex members affect the ability of SIN3A to bind to chromatin. The complex components effect on cell proliferation was also examined through clonal analysis of imaginal wing discs. The results of this work implicate a role of several members of the SIN3A complex for proper recruitment and localization to chromatin. All of the SIN3A complex members had some varying effect on cell proliferation, much like that of SIN3A. This study provides a better understanding of SIN3A-RPD3 complex members regarding how they might be influencing SIN3A function in Drosophila melanogaster.
Laity, Kelly Ann, "The Phenotypic Analysis Of The Knockdown Of The Sin3a Complex Components And Their Role In Recruitment And Cell Proliferation" (2014). Wayne State University Theses. 348.