Optimization Of Labeling Techniques; Determination Of Best Parameter For Olfactory Mucosal Progenitor Cell Delivery And Study Of Effects Of Methylene Blue And Polyethylene Glycol In An Animal Model Of Spinal Cord Injury
Open Access Thesis
Date of Award
Biochemistry and Molecular Biology
Jean P. Nelson
In the United States, the incidence of TSCI is about 40 per million persons per year, with approximately 250,000 living survivors of TSCI in the United States in July 2005. A number of human clinical trials (85) are ongoing using stem cells to evaluate the methods to reduce the injury following TBI. Among the stem cells, olfactory mucosal progenitor cells have several advantages with respect to ease of obtaining, fate of cells, etc. Our study was intended to optimize the labeling technique and to determine the best parameter to deliver the stem cells to the site of injury. We found that CFDA when used at 5 µM concentration, gives good results. We could successfully design an animal model for chemicals induced spinal cord injury using CTB-SAP. Immunofluorescence studies have shown that OMPCs have migrated to the area of injury from the area of injection.
We also aimed to study the effects of polyethylene glycol and methylene blue on the functional recovery following neuronal injury. Results were encouraging as there was significant functional improvement in BBB scores as a result of using a series of solutions that contain PEG and MB.
Rokkappanavar, Kiran Kumar, "Optimization Of Labeling Techniques; Determination Of Best Parameter For Olfactory Mucosal Progenitor Cell Delivery And Study Of Effects Of Methylene Blue And Polyethylene Glycol In An Animal Model Of Spinal Cord Injury" (2013). Wayne State University Theses. 274.