Access Type

Open Access Thesis

Date of Award

January 2013

Degree Type


Degree Name



Biochemistry and Molecular Biology

First Advisor

Bharati Mitra


Zinc is essential trace elements for all living organisms and serves as cofactor and structural cofactors for more than 300 enzymes. ZupT is one of the members of ZIP super family of transporter proteins, which were identified as iron and zinc transporter in Eukaryotes at first. However, recent evidence showed that some of members can also transport other metal ions, such as manganese or cadmium, and ZIP proteins also exist in bacteria. ZupT is responsible for zinc uptake in E. coli; however, its full metal selectivity is not yet characterized. In addition, the mechanism of transport is also unknown. Therefore, my goal is to check the affinity of ZupT with different metal ions, and try to define the metal binding site by testing different mutants.