Open Access Thesis
Date of Award
Nutrition and Food Science
Smiti V. Gupta
Oil palm phenolics, a water soluble portion is obtained from the milling of palm oil. It has been proven to be a remarkable anti-oxidative agent which reduces the overall oxidative stress in the body and cardiovascular disease in mice. The aim of this study was to see whether OPP has any beneficial effects on the development or progression of pancreatic cancer. A total of 30 male LSL.KrasG12D/+; p53R172H/+; PdxCretg/+ were categorized into control (CP, KC=6) and treatment (KP, KG, KPG=6) groups. The control groups received the regular chow dietand the treatment groups revceived 5%OPP chow diet. 5µg of saline was give every alternate day and the treatment groups KP and KPG received the chemotherapeutic drug, Gemcitabine. After the six week long study, the animals were sacrificed, tissues collected and stored in 10% formalin for histological studies. Hematoxylin & Eosin staining was done to study the epithelial lesions caused by the cancerous cells in the exocrine pancreatic tissue. Immunohistochemistry using DPC4/SMAD4 & S100P antibodies was also done to observe the DNA damage of each mouse. The study shows the positive synergistic correlation of OPP and Gemcitabine together which reduce the overall incidence of pancreatic adenocarcinoma in the KPC mice
Gowthaman, Poornima, "Anti-Cancer Effects Of Oil Palm Phenolics On Pancreatic Cancer - Histological Evidence" (2013). Wayne State University Theses. 264.