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Access Type

WSU Access

Date of Award

January 2011

Degree Type


Degree Name




First Advisor

Mary K. Pflum


Protein phosphorylation and nucleotidylation are two important post-translation modification on proteins. They have many functions in cell signaling and regulation of gene expression. Therefore it is crucial to understand their behaviour and activity. Several tools were developed to study these two modifications in this thesis. In the first part, phosphorylation of proteins is catalyzed by kinases and the reverse dephosphorylation reaction is catalyzed by phosphatases. Proteins were biotinylated via phosphorylation using ATP-Biotin. The sensitivity of biotinylated phosphoproteins to phosphatases was studied. In the second part, a novel and convenient organic synthetic route was proposed to prepare nucleotidyl phosphorylated peptide. Solid Phase Peptide Synthesis and on resin nucleotidylation were investigated. In conclusion, several novel tools to study protein phosphorylation and nucleotidylation were developed in this thesis.

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