Open Access Dissertation
Date of Award
Nutrition and Food Science
Diane C. Cabelof
Folate is an essential nutrient for human. They carry and transfer one carbon units. They has important biological functions during many reactions, like DNA synthesis, RNA synthesis, DNA methylation, DNA repair, cell division and growth. Through biochemical techniques to determine the gene expression, enzyme activity, uracil level in DNA, and through microbiological assay to determine the folate level, elucidates mechanism of uracil accumulation response to folate depletion. The findings present folate deficiency status affects the uracil DNA glycosylase activity, and uracil initiated DNA base excising repair. DNA repair ability decide whether there is uracil accumulation under the folate depletion condition in different cell lines. Further, there is uracil accumulation in the mice colon tissue found under the aging condition.
Ma, Hongzhi, "Uracil Accumulation Response To Folate Depletion Is A Function Of Dna Repair Imbalance That May Be A Mechanism Of Aging" (2014). Wayne State University Dissertations. 969.