Open Access Dissertation
Date of Award
As high-throughput gene expression data becomes cheaper and cheaper, researchers are faced with a deluge of data from which biological insights need to be extracted and mined since the rate of data accumulation far exceeds the rate of data analysis. There is a need for computational frameworks to bridge the gap and assist researchers in their tasks. The Topology Enrichment Analysis frameworK (TEAK) is an open source GUI and software pipeline that seeks to be one of many tools that fills in this gap and consists of three major modules. The first module, the Gene Set Cultural Algorithm, de novo infers biological networks from gene sets using the KEGG pathways as prior knowledge. The second and third modules query against the KEGG pathways using molecular profiling data and query graphs, respectively. In particular, the second module, also called TEAK, is a network partitioning module that partitions the KEGG pathways into both linear and nonlinear subpathways. In conjunction with molecular profiling data, the subpathways are ranked and displayed to the user within the TEAK GUI. Using a public microarray yeast data set, previously unreported fitness defects for dpl1 delta and lag1 delta mutants under conditions of nitrogen limitation were found using TEAK. Finally, the third module, the Query Structure Enrichment Analysis framework, is a network query module that allows researchers to query their biological hypotheses in the form of Directed Acyclic Graphs against the KEGG pathways.
Judeh, Thair, "Teak: A Novel Computational And Gui Software Pipeline For Reconstructing Biological Networks, Detecting Activated Biological Subnetworks, And Querying Biological Networks." (2014). Wayne State University Dissertations. 965.