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Access Type

WSU Access

Date of Award

January 2020

Degree Type

Dissertation

Degree Name

Ph.D.

Department

Biomedical Engineering

First Advisor

Weiping . Ren

Abstract

Amorphous calcium polyphosphate (ACPP) is an inorganic polymer ceramic. Here we use a simple method of preparing ACPP hydrogel in the presence of excess volume of water. Essentially, water availability to polyphosphate chains accelerates water molecule ingress and microstructural transformation of ACPP hydrogels. Antibiotic delivery capacity of ACPP hydrogel increases by the specific mixing and molding method, where the VCM has higher antibiotic encapsulation efficiency with the small burst release for the compressed discs. In part of this study, we investigate impact of ACPP hydrogel replacement by monomeric calcium phosphate on the quality of final product (dicalcium phosphate dihydrates (DCPD) cement). Moreover, antibacterial activity of Polymeric Dicalcium Phosphate Dihydrate (P-DCPD) cement is one crucial step toward determining material viability for avoiding the bacterial growth. Thus in vitro bactericidal experiment shows the potency of P-DCPD material and selected antibiotics to invade bacteria and the correlation of the results with the antibiotic activity results from ACPP hydrogel discs.. For further advancement of P-DCPD material, an in vitro and in vivo biocompatibility study of the cement particles reveals the effect of physicochemical properties of the cement on cellular and tissue viability. We believe that the formation of ACPP hydrogel is through the mechanisms of intermolecular ionic interaction and entanglement of polyphosphate chains may affect drug retention within the matrix in a favorable way. As such, some of the surface physiochemical properties such as cement cohesion may affect cellular and tissue response. P-DCPD shows similar or even superior biocompatibility than classical/monomeric DCPD. In the future, the drug-eluting capacity of polymeric DCPD will be investigated to correlate it with the obtained results from ACPP hydrogel.

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