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We studied five population samples from Argentina, four drawn from Native American groups of the northeast region (Wichı, Pilaga, Toba, and Mbya-Guaranı) and one from two small villages of the Cordoba province. In this study we report genotypes and allele frequencies of the 9.1-kb insertion-deletion polymorphism located on chromosome 22. The frequency of the deletion allele ranges from 0.276 in the Mbya-Guarani to 0.470 in the Pilaga. The coefficient of population differentiation is fairly low (FST = 0.013), does not reflect any geographic or linguistic pattern, and seems to be more related to stochastic processes than to directional forces.