Allele Frequencies of Single Nucleotide Polymorphisms in the Second Exon of the Myoglobin Gene among the Japanese

Document Type

Article

Authors

Tomoyo Takata, Department of Legal Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Satoru Miyaishi, Department of Legal Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Yuji Yamamoto, Department of Legal Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Sachiyo Inagaki, Department of Legal Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Kei Yoshitome, Department of Legal Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Takaki Ishikawa, Department of Legal Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Hideo Ishizu, Department of Legal Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.

Abstract

The difference in the allele frequencies of two single nucleotide polymorphisms (SNPs) in the second exon of the myoglobin gene between Japanese and other populations is reported. These SNPs are the substitutions of (A79G) and (T109C), and they were investigated by a single polymerase chain reaction–single strand conformation polymorphism (PCR-SSCP) analysis followed by direct sequencing. The substitutions were always linked and two alleles were found in the samples used: the A-T allele with no substitution at positions (79A) and (109T) and the G-C allele with substitutions of (79G) and (109C). The frequencies of these alleles were 0.755 and 0.245, respectively, and they were found to be in Hardy-Weinberg equilibrium. The distribution of alleles in the Japanese population was significantly different from that reported among whites, blacks, and Hispanics (p < 0.0001).

Recommended Citation

Takata, Tomoyo; Miyaishi, Satoru; Yamamoto, Yuji; Inagaki, Sachiyo; Yoshitome, Kei; Ishikawa, Takaki; and Ishizu, Hideo (2002) "Allele Frequencies of Single Nucleotide Polymorphisms in the Second Exon of the Myoglobin Gene among the Japanese," Human Biology: Vol. 74: Iss. 2, Article 11.
Available at: https://digitalcommons.wayne.edu/humbiol/vol74/iss2/11