Off-campus WSU users: To download campus access dissertations, please use the following link to log into our proxy server with your WSU access ID and password, then click the "Off-campus Download" button below.
Non-WSU users: Please talk to your librarian about requesting this thesis through interlibrary loan.
Date of Award
Donald V. Coscina
The corticotropin releasing factor (CRF) system is involved in the regulation of feeding and metabolism. Central administration of Ucn 1, a non selective CRF-R agonist, has been shown to produce immediate short-term feeding suppression and lowered respiratory quotients (RQs) in rats. The latter indicates a shift toward preferential oxidation of fatty acids. Centrally administered Ucn 2, a selective CRF-R2 agonist, produced delayed onset feeding suppression. The present study tested the effect of intracerebroventricular administration of Ucn 2, a CRF-R1 antagonist, and a CRF-R2 antagonist on RQ, energy expenditure (EE) and post-test food intake. These variables were also measured after animals were pre-treated with either a CRF-R1 or CRF-R2 antagonist prior to injection of Ucn 2.
Ucn 2 suppressed RQs, which indicated a greater shift toward fat metabolism than would otherwise occur under normal fasting conditions. Ucn 2 did not reliably alter EE or post-metabolic test food intake. Astressin 2-B, a CRF-R2 antagonist did not reliably alter RQ, EE or post-test food intake. The low 300 pmol dose of R121919, a CRF-R1 antagonist, did suppress RQ but had no effect on EE or post-test food intake. The higher doses of R121919 did not alter RQ, EE or post-test food intake. Pre-treatment with either antagonist blocked the effect of Ucn 2 to suppress RQ. These results, taken along with previous research showing Ucn 2 suppresses food intake, suggests that this peptide has the potential to be used as a pharmacological treatment for obesity.
Laplante, Kimberly Anne, "Urocortin 2, A Corticotropin Releasing Factor Receptor 2 Agonist, Alters Energy Substrate Utilization" (2010). Wayne State University Theses. Paper 49.