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Access Type

WSU Access

Date of Award

January 2015

Degree Type

Thesis

Degree Name

M.S.

Department

Nutrition and Food Science

First Advisor

Diane C. Cabelof

Abstract

ABSTRACT

THE EFFECT OF DIETARY FOLATE ON TISSUE FOLATE STATUS.

by

Ghada Aoun

December 2015

Advisor: Dr. Diane Cabelof

Major: Nutrition & Food Science

Degree: Masters of Science

The folates are a family of structurally similar, water soluble, B vitamins that play a crucial role in human health and disease. Folate has an important role in the synthesis of DNA, DNA methylation, and cellular growth. Folate coenzymes function in accepting and donating one-carbon units in the folate metabolic pathway. Folate deficiency impairs DNA synthesis, induces cancer, and causes abnormalities such as neural tube defects and megaloblastic anemia. While scientific research has established the effects of folate deficiency on serum folate, a comprehensive evaluation of the effects of folate deficiency in tissues and cells has yet to be conducted. Using molecular techniques to determine gene expression and a microbiological assay to determine the folate level, we elucidated the mechanism of folate tissue status in response to folate depletion. Our study showed that dietary folate deficiency had an impact on serum folate as well as tissue folate status. This demonstrates the differential effect of folate deficiency on individual health and gene expression.

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