Access Type

Open Access Thesis

Date of Award

January 2013

Degree Type

Thesis

Degree Name

M.S.

Department

Nutrition and Food Science

First Advisor

Smiti V. Gupta

Abstract

Oil palm phenolics, a water soluble portion is obtained from the milling of palm oil. It has been proven to be a remarkable anti-oxidative agent which reduces the overall oxidative stress in the body and cardiovascular disease in mice. The aim of this study was to see whether OPP has any beneficial effects on the development or progression of pancreatic cancer. A total of 30 male LSL.KrasG12D/+; p53R172H/+; PdxCretg/+ were categorized into control (CP, KC=6) and treatment (KP, KG, KPG=6) groups. The control groups received the regular chow dietand the treatment groups revceived 5%OPP chow diet. 5µg of saline was give every alternate day and the treatment groups KP and KPG received the chemotherapeutic drug, Gemcitabine. After the six week long study, the animals were sacrificed, tissues collected and stored in 10% formalin for histological studies. Hematoxylin & Eosin staining was done to study the epithelial lesions caused by the cancerous cells in the exocrine pancreatic tissue. Immunohistochemistry using DPC4/SMAD4 & S100P antibodies was also done to observe the DNA damage of each mouse. The study shows the positive synergistic correlation of OPP and Gemcitabine together which reduce the overall incidence of pancreatic adenocarcinoma in the KPC mice

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