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Date of Award
GPI anchors are a complex class of glycolipids that serve to localize a wide range of extracellular proteins and glycoproteins. The reason that nature uses the GPI anchor as opposed to simpler lipid modifications is essentially unknown. This renders the need to study structure/activity relationships (SAR) of GPI anchors. Due to structural microheterogeneity and low natural abundance, obtaining pure and intact GPI anchors from natural sources is practically impossible. These types of SAR studies require access to pure and structurally well defined GPI anchors and GPI anchor analogs, leaving the challenging chemical synthesis as the most practical means of obtaining GPI anchors for study. This thesis will focus on the development of a synthetic strategy for obtaining a GPI derivative bearing a fluorescent tag and an unsaturated lipid chain. The target GPI derivative is intended for future biological studies that will look at its behavior on an extracellular surface.
Johnson, Charles Lee, "Synthetic studies toward a glycosylphosphatidylinositol (gpi) anchor derivative containing an unsaturated lipid chain and a fluorscent tag" (2011). Wayne State University Theses. 125.