Access Type
Open Access Dissertation
Date of Award
January 2017
Degree Type
Dissertation
Degree Name
Ph.D.
Department
Physiology
First Advisor
Donald J. DeGracia
Abstract
This Dissertation is the first to estimate the variables D and S of the nonlinear theory of acute cell injury in hippocampal regions CA1 and CA3 using a rat model of global brain ischemia and reperfusion (I/R). D was estimated by quantifying protein aggregates using ubiquitin Western blotting. S was estimated by quantifying changes in polysomal mRNAs as measured by microarray chips. D and S time courses were sampled at 0.5, 8, 16, 24, 48, and 72 hrs of reperfusion after 10 min brain ischemia. CA1 dies and CA3 survives. The theory predicts D will be larger in CA1 and S in CA3. Area under the curves of the D and S time courses showed this to be true. Trajectories of D vs S qualitatively matched updated versions of the theory. However, quantitative fits gave nonlinear correlation coefficients in the range 0.7-0.8. It was concluded that the acquired data did not falsify the theory. However, inadequate sampling over the time course, and the underestimation of S by failing to account for non-transcription dependent stress responses prevented strong quantitative fits between the measured estimates of D and S and theoretical time courses.
Recommended Citation
Anggraini, Fika Tri, "Estimating The Variables Of The Nonlinear Dynamical Theory Of Acute Cell Injury" (2017). Wayne State University Dissertations. 1779.
https://digitalcommons.wayne.edu/oa_dissertations/1779